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A 96-well plate in which two of the rows have been coated with a polymer mixed with a fluorescing dye to demonstrate the uniformity of the coatings.
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Various laboratories, including ours, have created libraries of polymers with a range of properties for use in biomedical applications. However, high-throughput evaluation of the polymers within the library has been a constraint on rapid screening of these polymers for specific applications. To meet this need, we have developed a technique to provide a large number of polymers in a convenient 96-well format for use by other laboratories. The plate consists of 12 polymers coated in a 96-well polypropylene plate in such a way that the entire well including the wall is covered with the polymer coating. This prevents any confounding of the cell culture results from polypropylene wall surfaces of the underlying plate, which might result if only the bottom of the wells is coated. This should add confidence to the conclusions drawn from in-vitro testing related to the effect of polymeric biomaterials on cell functions.
Cell growth (HDF- Human Dermal Fibroblast) and static contact angle of a methacrylate library of polymers |
We recently completed, in collaboration with Prof. Julia Babensee of GA Tech, a screening of the polymethacrylate library in the context of dendritic cell phenotype and intend to present the data in the upcoming Society for Biomaterials meeting in Seattle, WA in 2010. Further testing of hMSCs (human Mesenchymal Stem Cells) is underway in the RESBIO program to understand how biomaterials might modulate hMSC self-renewal and differentiation. We are also conducting in-house testing using other cell lines to demonstrate the general utility of this testbed so that a wide range of researchers working in different areas of biomedical engineering, drug delivery, tissue engineering and regenerative medicine can benefit from this platform methodology. We invite you to test this high-throughput testbed to see if you can accelerate the pace of discovery of your research efforts also. Please contact Dr P. Narayanan at pnarayan@biology.rutgers.edu for further information. Page last updated: December 2nd, 2009
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