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Cells on Polymeric Biomaterials – A short course offered by RESBIO, Technology Resource for Polymeric Biomaterials
Profs. Prabhas Moghe (left) and Pallassana Narayanan (front) with attendees of the Cells on Polymeric Biomaterials short course, October 29, 2009.
2010 Short Course dates will be announced by March
The Course Overview Cells on Polymeric Biomaterials is an in-depth 3-day short course designed for scientists and engineers to gain an understanding of how cells and proteins interact with materials. Scientific personnel and managers of interdisciplinary teams involved in tissue engineering or regenerative medicine, and anyone whose research touches on cell-material interactions, can benefit from instruction in novel approaches to accelerate the pace of discovery. The anticipated outcomes are better design of new medical implants and improved performance of existing devices. Significant emphasis will be placed on hands-on training so that participants can evaluate the available RESBIO resources and continue to use these resources for their needs even after the course.
Three major topics on materials, confocal microscopy and computational modeling will be covered with one day allocated for each topic. The format of each day will be morning lectures and afternoon hands-on training. The materials topic will cover synthesis, fabrication and characterization of polymeric biomaterials. Perspectives on synthesis, processing and characterization in the context of product and process development, validation and regulatory submission will be presented. The confocal microscopy section will focus on cells-material sample preparation, imaging and analysis. The computational modeling course introduces the public interested in modeling of polymeric biomaterials to the state-of-he art molecular modeling tool Biomaterials StoreTM, developed at Rutgers Center for Computational Design and New Jersey Center for Biomaterials. The workshop will provide an introduction to the tools available in Biomaterials StoreTM and also a hands-on session where the users will work through a tutorial on the software. Click here for more details.Course Outline
| Day 1 |
Polymer Synthesis, Substrate Fabrication and Material Characterization, 9 AM - 5 PM
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| Morning: |
Combinatorial Computational Approach Design of Polymer Libraries Parallel Synthesis of Polymer Libraries
Fabrication of Implants/Scaffolds for Studying Cell Behavior and Water Uptake Filament/Fiber Extrusion Electrospinning Casting and coatings Compression and Injection Molding
Material Characterization for Structure-property-behavior Correlations QCMD – Protein adsorption on biomaterials DSC, TGA – Degradation and water uptake Mechanical – Load bearing and deformation HPLC / GPC – Molecular weight NMR – Composition for validation of synthesis step Microscopy (Optical, SEM, TEM) – Surface
characterization
PCR – Gene expression and regulation, receptor
up/down regulation
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| Afternoon: |
Hands-on training and demonstrations of synthesis, fabrication and characterization of polymeric biomaterials and scaffolds |
| Day 2 |
Imaging of the Interactions of Cells on Biomaterials, 9 AM - 5 PM
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| Morning: |
Overview of Confocal / Multiphoton Microscopy Theory and applications: Confocal/multiphoton
white light/epi-fluorescent
Equipment: Leica Imaging Suite Operation: Basic protocols Sample Preparation: 2D substrate, scaffold
preparation
Imaging and Interpretation Equipment tour
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| Morning & Afternoon: |
Hands-on Training on Sample Preparation and Imaging 3D imaging-based scaffold microsctructure
characterization
Multimodal imaging of cells on 2D films & 3D
scaffolds
Cell seeding and imaging on 3D biomaterials High-throughput imaging of cells on 3D
scaffolds.
Setup of time lapse studies to track tagged /
fluorescently engineered cells
Viral engineering of primary cells |
| Day 3 |
Semi-empirical Modeling of Bioresponse using Biomaterials StoreTM, 9 AM - 5 PM
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| Morning: |
Overview Theory of semi-empirical modeling Biomaterials StoreTM Part I: MySQL Biomaterials StoreTM Part II: WEKA |
| Afternoon: |
Computer Lab – Modeling hands-on training |
Registration:Industrial Participants:
$600 for the entire course $200, daily rate
Academic Participants:
$400 for the entire course
$150, daily rate
Those participants, who do not wish to attend all three days but choose to attend selected topics only, can register at the daily rate indicated above.Cancellation Policy: The full amount paid minus a $50 processing fee will be refunded for cancellations received prior to October 14, 2009. No refund will be issued for cancellations received after October 14, 2009. Late requests for refunds due to an emergency only will be considered on a case-by-case basis.All cancellations and requests for refunds MUST be submitted in writing via e-mail, fax, or mail by October 14, 2009. Telephone requests will not be honored. Short Course LocationNJ Center for Biomaterials 145 Bevier Road Piscataway, NJ 08854
Accommodation
Questions, ContactPallassana Narayanan PhDNJ Center for Biomaterials, Rutgers University,Chief Operating Officer, RESBIO145 Bevier RoadPiscataway, NJ 08854Phone: 732-445-0488 ext 40021Fax: 732-445-5006Email: pnarayan@biology@rutgers.eduWebsite Linkhttp://www.njbiomaterials.org/web/index.php?p=resbio
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