Cell Biology & Cell-Material Interactions

The New Jersey Center for Biomaterials (NJCBM) Cell Biology and Cell Culture Laboratory was established in September 1998 in the Wright-Rieman Laboratories at Rutgers University in Piscataway, NJ to provide cell biology laboratory resources dedicated to in vitro biomaterials research. In 2005, the laboratory was moved to an expanded space in the Center's new home, the adjacent Life Sciences Building.  The new laboratory operates a Class 10,000 clean room and is equipped to support research in cell biology, cell culture and manipulation, cell-material interactions, nucleic acid and protein analysis. The laboratory is also associated with a microbiology facility which supports culture and manipulation of bacteria and viruses.

Facilities

  • A class 10,000 clean room facility for routine cell culture, cell and molecular biology and biomaterial research.
  • Four Baker SterilGARD II biosafety cabinets equipped with gas and vacuum lines for optimal sterility and handling of biological and non-biological materials.
  • Two Forma Scientific Cell Culture incubators for constant temperature, humidity, and carbon dioxide conditions.
  • Olympus inverted and reflected light microscopes equipped with a 6 MP digital camera.
  • Liquid nitrogen dewars equipped with a monitoring system for secured storage of frozen cells.
  • Two -80°C freezers, one  -20°C freezer and one -4°C refrigerator for a range of storage conditions.
  • Nanopure water filtration systems.
  • Beckman refrigerated table-top ultracentrifuge, regular centrifuge and Eppendorf micro-centrifuge for various centrifugation needs.
  • Molecular Devices SPECTRAmax Gemini II Fluorescence and Bio-Tek Powerwave X absorbance plate reader for high throughput assays.
  • Perkin Elmer PCR thermal cycler for DNA and RNA analysis.
  • Gel electrophoresis setup and a Syngene bioimaging system for gel imaging, documentation and quantification.
  • Real-time RT-PCR
  • Beckman Ultracentrifuge
  • 3d printers listed in NJCBM Annual Report (new one to be added soon)